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After most of Spain's World Cup-winning squad were selected for upcoming games earlier on Monday, the players said they would continue their boycott, which came after Rubiales kissed Hermoso on the lips during the trophy presentation ceremony.
The order prevents Rubiales trying to contact Hermoso or coming within 200 metres of her.
The players, who are not minors, were later released following a court decision and after the data on their mobile phones was seized.
Rubiales announced his resignation in a statement in which he said his position had become untenable, with the Spanish Football Federation later confirming he had sent a resignation letter to acting president Pedro Rocha.
Prosecutor Marta Durantez Gil's decision came three days after Hermoso lodged a criminal complaint. It is up to the court to present formal charges.
In other qualifiers, Paraguay drew 0-0 with Peru while Colombia claimed a 1-0 home win over Venezuela.
The national prosecutor's office said Spain's football federation boss Luis Rubiales could face criminal charges in addition to the ongoing 'serious misconduct' enquiry.
Montse Tome, who was Vilda's assistant since 2018, becomes the first woman to helm the women's national team.
Alejandro Blanco has said Rubiales' gesture cannot be tolerated.
Spanish football federation president Luis Rubiales' kiss of Jenni Hermoso not only soured the World Cup triumph, but has also triggered a torrent of allegations from other women in sport of predatory behaviour by men.