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Barca's all-time top scorer was seen smiling as he and his fellow players worked through persistent rain at the club's Joan Gamper training complex under the orders of new coach Ronald Koeman.
A day after the Spanish club reaffirmed its position not to facilitate a transfer, Messi's father-agent did not dismiss the possibility of his son reconsidering his decision to leave.
Real Madrid clinched a record-extending 34th La Liga title with one game to spare by beating Villarreal 2-1 at home.
Real will clinch their first league title since 2017 with a win over Villarreal on Thursday or at Leganes on the final day of the season.
Chilean midfielder Arturo Vidal struck the only goal of the game in the 15th minute, firing into the net off the post after being played in by Messi.
Barca, chasing a third consecutive Spanish title, are on 76 points after 35 games while Real Madrid are top with 77 and can restore their four-point advantage when they host Alaves on Friday.
Barcelona president Josep Maria Bartomeu has said that captain Lionel Messi will end his career at the Catalan club, dismissing a report saying the Argentine no longer wishes to renew his current contract when it expires next year.
The Catalans had drawn their last two matches to hand more momentum to league leaders Real Madrid but Sunday's win took them to 73 points after 34 games, four behind their arch-rivals.
The hard-fought win took Zinedine Zidane's side on to 77 points after 34 games, seven clear of second-placed Barca.
Barca edged out Athletic Bilbao 1-0 as substitute Rakitic scored the winner off an assist by Messi.