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The visitors skittled out England for 149 to register victory by an innings and 12 runs.
South Africa will not call up a replacement despite fellow seamer Kagiso Rabada being in a race to recover from an ankle problem.
India levelled the five-match series 2-2 after the visitors lost the fourth T20I by 82 runs.
Harshal Patel (4/25) and Yuzvendra Chahal bagged (3/20) helped India make it 2-1 in five-match series.
Klaasen scored 81 off 46 deliveries to help the visitors post a 4-wicket win.
The Proteas have handed a maiden Twenty20 International call-up to batsman Tristan Stubbs, for the five-match tour of India starting on June 9.
Invited to bat, DC posted 159/7, courtesy Mitchell Marsh's 48-ball 63, and then restricted Punjab to 142/9.
Punjab have made a change while RCB are unchanged from their previous match.
Dhawan scored his third half-century of the season to script an 8-wicket win.