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Osaka, who last played competitively when she lost to Leylah Fernandez in the third round of the US Open in September, displayed plenty of her trademark power but often lacked accuracy at the Australian Open warm-up event.
Pliskova is the latest high-profile player to miss the Melbourne Park major, with Grand Slam champions Serena Williams, Bianca Andreescu and Roger Federer also absent.
Amnesty International's China researcher Alkan Akad told Reuters the video call did little to ease fears over Peng's wellbeing.
In a statement, the IOC said Peng began the 30-minute call with its president Thomas Bach by thanking the Olympic organisation for its concern.
Peng 35, a two-time former Grand Slam doubles champion, has vanished from the public eye ever since she accused former Chinese vice-premier Zhang Gaoli, 75, of coercing her into sex at his home in a since-deleted social media post on November 2.
Former doubles world No. 1 Peng has not been seen or heard from publicly since she said on Chinese social media on November 2 that the former vice-premier, Zhang Gaoli, coerced her into sex and that they later had an on-off consensual relationship.
Peng, one of China's biggest sport stars, said on social media this month that former Chinese vice-premier Zhang Gaoli coerced her into sex and that they later had an on-off consensual relationship.
Osaka, who turned 24 on Saturday, posted several pictures of her on social media.
With this new technique, the Japanese pharmaceutical group aims to break into a market which is dominated by major drugmakers Pfizer, Moderna and AstraZeneca.
Osaka, 23, slipped from seventh to 12th position, ending her streak of 140 consecutive weeks in the top 10.