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Some parliamentarians appeared dissatisfied with the salary hike and instead sought an increase in the MP Local Area Development Fund (MPLADS).
Opposition party members rushed into the well of the House as the IT minister was called to make a statement over the issue.
Chairman M Venkaiah Naidu also rejected a notice for no confidence motion against deputy chairman Harivansh on the grounds that proper procedure was not followed.
Ghulam Nabi Azad said they would boycott the proceedings till the suspension of members is revoked and also demanded that the government bring another bill to ensure that no private player should buy food grains from farmers below the MSP fixed by the government.
The mandatory tests were conducted ahead of the Monsoon session of Parliament, which began Monday.
Rajya Sabha Deputy Chairperson Harivansh on Tuesday announced a 24-hour long fast, hoping that this may inspire a feeling of 'self-purification' in the opposition.
The MPs were suspended on Monday, a day after the Upper House witnessed unprecedented unruly scenes by protesting opposition members during the passage of the two bills, described as the biggest reform in agriculture by the government.
The Kerala High Court has said settlement of cases are getting delayed because the accused are not being arrested and produced in court.
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