The veteran athlete said doping was a serious issue in the country that needs to be addressed.
CPM's V Sivadasan and A A Rahim and CPI's Santhosh Kumar are among those who were suspended.
The iconic former track and field athlete has maintained that she will continue to work for sports.
The opposition has raised their voice against a recent booklet issued by Lok Sabha Secretariat listing a host of words to be disallowed in the Parliament.
BJP Kerala has also claimed there was no political motive behind Usha's nomination by the Narendra Modi government.
The Rajya Sabha was modelled on the House of Lords, as an upper chamber that would not be elected by popular vote but consist of elder statesmen appointed on the basis of their stature and credentials.
Big-wigs Nirmala Sitharaman and Piyush Goyal, Randeep Surjewala and Jairam Ramesh of the Congress and Shiv Sena's Sanjay Raut among 16 candidates elected.
Elections will be held for 16 seats of Haryana, Rajasthan, Maharashtra and Karnataka on Friday as the number of candidates exceeds the seats going to the polls.
He told the media that the resignation had been given on May 16 itself and that it was imperative the Parliament had independent voices.
Priyanka's grandmother the late Indira Gandhi and mother Sonia Gandhi have earlier contested elections from Karnataka and emerged victorious.