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Special Judge Geetanjali Goel granted relief to the accused after they appeared before the court in pursuance of summons issued against them.
Prasad's arch rival-turned-ally, Bihar Chief Minister Nitish Kumar, meanwhile, said he is looking forward to holding a meeting of opposition leaders after formation of a new government in the southern state.
The ED said the investigation is underway to unearth more investments made on behalf of Prasad's family and their associates in various sectors, including real estate, at various places.
The agency has kept the probe open into the alleged scam and the new round of questioning of Yadav family members is in connection with 'further investigation' in the case, they said.
A chargesheet was filed last October against Yadav, his wife Rabri Devi and 14 others.
The scam involved taking land from unemployed youths on the pretext of providing them government jobs.
A special CBI court in Ranchi on Monday sentenced the Rashtriya Janata Dal chief to five years imprisonment and imposed a fine of Rs 60 lakh on him in the Rs 139.35 crore Doranda treasury scam, the fifth fodder scam case.
Prasad, who has already been convicted in four other cases of the fodder scam, is also an accused in the fifth and final case.
The court directed him neither to leave the country without permission nor change his address and mobile number during the bail period.
The former Chief Minister of Bihar, Prasad is undergoing treatment a private hospital in Rachi for multiple ailments.