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The Netherlands finished with 23 points from 10 games, two more than Turkey, who won 2-1 away to Montenegro to secure a play-off spot.
The Italians were held to a 0-0 draw away to Northern Ireland and must now play in March's play offs.
Portugal, who only needed a draw with Serbia to finish top on goal difference and qualify, will now head into the March play offs.
Holders France ran riot to reach the finals with a match to spare after four goals from Kylian Mbappe helped them to an 8-0 home demolition of Kazakhstan.
The three points take Argentina on to 28 points, six behind Brazil, who on Thursday became the first South American team to guarantee their place in Qatar 2022.
The win extended Brazil’s home run in the qualifiers to 11 consecutive victories, a record in the South American region.
Peru missed a second-half penalty in a 1-0 loss to Argentina in Buenos Aires.
A second-half double by Timo Werner and one goal each from Kai Havertz and Jamal Musiala secured Germany's fifth win in their fifth game under new coach Hansi Flick.
The results leave Brazil in top spot with 28 points, six ahead of Argentina.
Pele had been hospitalised for nearly a month, checking in for routine exams before undergoing a September 4 operation to remove the tumor.
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