It is a 44-minute-long series presented in six parts by celebrity chefs Gari Mehigan and Pablo Naranjo Agular.
Ayyanthole secured prizes in four other categories including Best Drummers.
The two women will perform for the Sitaram Mill Desham that is making a comeback after 11 years.
This year, all five teams have 51 tigers each. The "tigresses" are from Viyyur Desam.
This is the 16th year that a giant Pookkalam was arranged in front of Sree Vadakkumnathan Temple.
Five teams will take part in the event that will be held at Swaranj Ground, on the fourth day of the Onam festival.
Pulikali is a major component of the fourth day of Onam in Thrissur when men paint their potbellies with the shapes of tigers and prance around Swaraj Round every year.
India is host to many distinctive rituals, festivals and traditions that range from simple and sweet to hugely controversial at times.
The input restrictions of Covid-19 and the associated economic distress extend the threat of extinction over the art form.
Sixteen artists perform at separate locations and the visuals shared on a single Facebook page through the Zoom app