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A total of 32 people are currently under observation at Manjeri Government Medical College Hospital and Perinthalmanna MES Medical College Hospital.
At present, there are 267 persons in the contact list.
Health Minister Veena George said 20 persons on the contact list tested negative.
The condition of the patient is stable, said minister Veena George.
Field survey in Mampad, Thiruvali and Wandoor panchayats has covered 7,953 houses.
Kerala's robust surveillance of Acute Encephalitis Syndrome (AES) triggered an investigation into the death of a 23-year-old patient at a private hospital in Malappuram.
Till now, 16 samples have been tested negative.
Five wards in two panchayats of Malappuram have been declared containment zones.
Health Minister Veena George said samples from 13 individuals on the contact list have been sent for testing.
The youth visited the Nilambur Police Station, two local clinics and two hospitals between September 6 and 9.